Platelet Flow Cytometry: Instrument Setup, Controls, and Panel Performance
نویسندگان
چکیده
منابع مشابه
Flow cytometry controls, instrument setup, and the determination of positivity.
A frequent goal of flow cytometric analysis is to classify cells as positive or negative for a given marker, or to determine the precise ratio of positive to negative cells. This requires good and reproducible instrument setup, and careful use of controls for analyzing and interpreting the data. The type of controls to include in various kinds of flow cytometry experiments is a matter of some d...
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Platelet glycoprotein Ib (GpIb), a receptor for von Willebrand's factor (vWF), was studied by way of fluorescence flow cytometry. Using a sandwich staining technique, GpIb was identified by a monoclonal antibody (6D1) directed against an epitope close to the vWF binding site. Platelets from normal individuals were symmetrically distributed with respect to GpIb content. Treatment of washed plate...
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Flow cytometry enables studies of several different aspects of platelet function in response to a variety of platelet agonists. This can be done using only a small volume of whole blood, and also in blood with low platelet counts. These properties, together with the increasing number of flow cytometers available in hospitals worldwide, make flow cytometry an interesting option for laboratories ...
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Platelet glycoprotein lb (GpIb), a receptor for von Willebrand’s factor (vWF), was studied by way of fluorescence flow cytometry. Using a sandwich staining technique, Gplb was identified by a monoclonal antibody (6Di ) directed against an epitope close to the vWF binding site. Platelets from normal individuals were symmetrically distributed with respect to Gplb content. Treatment of washed plat...
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ژورنال
عنوان ژورنال: Cytometry Part B: Clinical Cytometry
سال: 2019
ISSN: 1552-4949,1552-4957
DOI: 10.1002/cyto.b.21774